Chemical & microbiological analysis of milk & milk products by Ramakant Sharma

By Ramakant Sharma

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The lysis of blood cells is due to the action of lytic agents produced by the organisms. For example, pyogenic streptococci and staphylococcus aureus elaborate powerful lytic enzymes, producing clear and wide zones around their colonies (Betahaemolysis). 2 Nutrient agar tubes. 4 Samples of milk freshly drawn aseptically from individual quarters of suspected animals for mastitis. 5 Defibrinated blood. 1 In a sterile flask containing glass beads defibrinate about 20-30 ml of rabbit, sheep or ox blood.

3 Sample bottles containing milk which are to be examined for flavour subsequently shall be closed with grease-proof, non-absorbent stopper so that no deleterious odour or taste is imparted. Note: Alternatively, for collecting samples for chemical analysis suitable plastic bottles of above capacities may also be used. 6 Sampling of Milk 9 Labelling of Samples - Each sample container shall be sealed air tight after filling and marked with particulars regarding the purpose of sampling, the name of the supplier or other particulars of the stock, the date and time of sampling, the nature of preservatives, if any, added and any other relevant information.

1 The testing laboratory, may, within its descretion, instruct the person who draws the sample to submit; (a) Separate samples from each unit selected, or (b) One or more composite samples consisting of aliquot portions from each unit selected. The latter course should only be applied where the product is likely to be of fairly uniform composition, for example, where the consignment to be sampled is produced from a quantity of properly bulked milks and where variations in composition from unit to unit are, therefore small.

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