Culture of Animal Cells: A Manual of Basic Technique and by R. Ian Freshney

By R. Ian Freshney

This is the main entire source at the strategies, apparatus, ideas, and practices of animal cellphone culture.

Since ebook of the former variation of this benchmark textual content, a variety of sorts of gear and fabrics have replaced and new really expert thoughts were built, rather within the sector of stem phone examine and tissue engineering. those and different advancements were integrated into this carefully revised and extended 6th version of Culture of Animal Cells, the major textual content within the uncomplicated method of phone culture.

The so much whole source at the suggestions, gear, ideas, and practices of animal cellphone tradition, this article bargains a whole history on the topic of development of animal cells in tradition. starting with laboratory layout, protection, validation and bioethics, then carrying on with with training of media, fundamental tradition and phone strains, via to characterization and authentication, illness, really good innovations, and troubleshooting, the assurance includes:
* An all-new component to education workouts, separated into uncomplicated, intermediate, and complex tactics, cross-referenced to the suitable protocols
* New assurance of stem cells, bioethics, validation, cloning, mobile signaling, in vitro toxicity trying out, and tissue engineering
* An extended full-color atlas part, with photos of basic tradition, phone strains, way of life, differentiation, melanoma cells and transformation, third-dimensional tradition, infection, and really good equipment
* better therapy of troubleshooting, with complete cross-referencing to the appropriate protocols and sections of text
* totally up to date references
* The clearest, such a lot constant presentation of step by step protocols available
* a number of diagrams, images, tables, and charts
* exact and up to date info on reagent training and sourcing of fabrics and gear, together with a completely up-to-date checklist of providers and different assets with net sites

Indispensable for scientific and biopharmaceutical researchers and scientists, scholars, trainees, and technicians, this landmark textual content provides the main available and entire advent on hand to the tradition and experimental manipulation of animal cells.

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Additional info for Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (6th Edition)

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2 Dedifferentiation Historically the inability of cell lines to express the characteristic in vivo phenotype was blamed on dedifferentiation. According to this concept, differentiated cells lose their specialized properties in vitro, but it is often unclear whether (1) the wrong lineage of cells is selected in vitro, (2) undifferentiated cells of the same lineage (Fig. 1), or (4) the differentiated cell reverts to a more primitive phenotype or even a stem cell. , 2004]. Also glial precursors, previously thought to be committed to become oligodendrocytes (OPCs), can be induced to revert to a common neural stem cell by treatment with BMP-2, BMP-4, and FGF-2, then reinduced to differentiate into either glia or neurons [Kondo & Raff, 2000].

2000], until the cell density reaches confluence, whereupon directional migration ceases. 2) and leads eventually to withdrawal of the cell from the division cycle. Myoblasts and endothelial cells migrate in a similar fashion and, like fibroblasts, may differentiate when they reach confluence, depending on the microenvironment. Epithelial cells, unless transformed, tend not to display random migration as polarized single cells. When seeded at CHAPTER 2 a low density, they will migrate until they make contact with another cell and the migration stops.

2001]. , 2003]. Tissue culture has also been used for diagnosis and toxicology. 4). In vitro toxicology has acquired greater importance in recent years due to changes in legislation regarding the usage of experimental animals, particularly in Europe. 2) are the ability to control the physiochemical environment (pH, temperature, osmotic pressure, and O2 and CO2 tension), which has to be controlled very precisely, and the physiological conditions, which have to be kept relatively constant. However, the physiological environment cannot always be defined where cell lines still require supplementation of the medium with serum or other poorly defined constituents.

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